BPC-157

BPC-157 is a synthetic 15-residue peptide, sequence GEPPPGKPADDAGLV, supplied by Alphex as a lyophilised powder in 5 mg and 10 mg vials for laboratory and in-vitro research. Each batch is tested by reverse-phase HPLC at an independent laboratory, and its certificate is published in our public COA library before the batch is listed. Stock status for each size is shown in the buy box on this page.

For research purposes only. This product is supplied exclusively for scientific laboratory research and analytical applications. It is not intended for human or animal use, and must not be misused in any way that contravenes regulations or applicable laws.

What BPC-157 is

BPC-157 is a pentadecapeptide, a chain of 15 amino acids: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. The sequence was described as a partial sequence of a larger protein isolated from gastric juice and named body protection compound, which is where the "BPC" comes from. The material sold for research is made by solid-phase peptide synthesis, not isolated from a biological source.

Chemically it is a short, hydrophilic peptide. The run of three prolines near the N-terminus gives that part of the chain a rigid shape, and the two aspartic acid residues and the glutamic acid give it a net negative charge at neutral pH. It dissolves readily in water. It is unusually stable for a linear peptide of this length, which is part of why it has been studied so often in solution-stability work.

Specification notes

The specification table further down this page lists the sequence, molecular formula (C62H98N16O22), molecular weight (1419.5 g/mol), CAS number and storage. Points to bear in mind:

• Mass basis. The 5 mg and 10 mg figures are the nominal fill of lyophilised material. They are not net peptide content. Synthetic peptides carry counter-ions and residual water, and Alphex certificates do not report net peptide content.
• Purity. The specification is 99+% by reverse-phase HPLC with UV detection: the share of the detected signal in the main peak.
• Appearance. White to off-white lyophilised powder or cake in a vacuum-sealed glass vial.

Where BPC-157 appears in the research literature

Most published BPC-157 work is preclinical. In the laboratory, the peptide is used in in-vitro assays such as cell-migration and scratch assays, endothelial tube-formation assays and studies of intracellular signalling pathways in cultured cells. It is also used in analytical work on peptide stability in different media. Our note on reading preclinical peptide literature critically explains how to weigh that body of work. Alphex supplies BPC-157 as a laboratory reagent only and does not provide protocols or application guidance.

Handling and storage in the lab

• Sealed vials. -20 C for long-term storage, 2-8 C for short-term storage. Let a cold vial reach room temperature before opening it to avoid condensation.
• Reconstitution. BPC-157 dissolves well in bacteriostatic water, sterile water or a neutral buffer. Add the diluent down the vial wall and swirl gently; do not shake.
• Stock solutions. Store at 2-8 C in the dark. For longer storage, aliquot and freeze at -20 C so each aliquot is thawed once.
• Concentration. Mass divided by volume. A 5 mg vial reconstituted with 2 mL gives a nominal 2.5 mg/mL stock. At 1419.5 g/mol that is about 1.76 mM.

Analytical notes

• Related impurities. In a 15-residue synthesis the most common impurities are deletion sequences, where one residue failed to couple, and truncated chains. The proline-rich N-terminal region can be harder to couple, so deletions there are worth looking for on a chromatogram.
• Detection. BPC-157 has no aromatic residues, so it is detected through the peptide bond at around 214 to 220 nm and absorbs very weakly at 280 nm.
• Identity. An HPLC purity figure shows how much of the detected material is in the main peak. Identity is evidenced separately, by retention against a reference standard or by another technique.

How the batch certificate works

Every Alphex batch is tested by Vanguard Laboratory, an independent analytical laboratory, using reverse-phase HPLC with UV detection. Vanguard is accredited to ISO/IEC 17025:2017 by A2LA under certificate 6377.01. The chromatographic purity result is not represented as an accredited-scope test.

The COA library holds the certificate for the most recent BPC-157 batch, vial label #0010-BPC. The laboratory recorded that submission under the sample marking "Red Cap" rather than the Alphex batch number, and the library notes this. That certificate reports purity of greater than 99.80% (plus or minus 0.18%) by HPLC-UV/VIS on a 5 mg vial. When a new batch is received, it is tested before it is listed and its certificate is added to the library. Type the code on your vial label into the library to find the matching certificate. The certificate is an RP-HPLC result; it does not report mass spectrometry, endotoxin, water content or net peptide content.

Vial sizes

Choose the vial by how much stock you will use within its useful life once reconstituted. Concentrations are nominal, based on vial fill rather than net peptide content.

Further reading

• BPC-157 research peptide: sequence, purity, storage and COA
• BPC-157 versus TB-500: the differences
• How to reconstitute research peptides: the step-by-step method

Alphex BPC-157 is supplied to laboratories and researchers for in-vitro research only. Delivery is UK only, by Royal Mail Tracked 24, which aims for the next working day.